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ATP1A3 Antibody

CSB-PA18139A0RB
Cusabio
ApplicationsELISA
Product group Antibodies
TargetATP1A3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATP1A3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID478
  • Target name
    ATP1A3
  • Target description
    ATPase Na+/K+ transporting subunit alpha 3
  • Target synonyms
    AHC2; ATP1A1; ATPase, Na+/K+ transporting, alpha 3 polypeptide; CAPOS; DYT12; Na(+)/K(+) ATPase alpha(III) subunit; Na(+)/K(+) ATPase alpha-3 subunit; Na+, K+ activated adenosine triphosphatase alpha subunit; Na+/K+ ATPase 3; RDP; sodium pump subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain; sodium/potassium-transporting ATPase subunit alpha-3; sodium-potassium ATPase catalytic subunit alpha-3; sodium-potassium-ATPase, alpha 3 polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13637
  • Protein Name
    Sodium/potassium-transporting ATPase subunit alpha-3
  • Scientific Description
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP1A3 using anti-ATP1A3 antibody (A02278-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A3 antigen affinity purified polyclonal antibody (Catalog # A02278-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A3 at approximately 90 kDa. The expected band size for ATP1A3 is at 112 kDa.
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