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Figure 1. Western blotting validation for Anti-ATP6V1A Antibody A10401 Western blot analysis of extracts of various cell lines
Figure 1. Western blotting validation for Anti-ATP6V1A Antibody A10401 Western blot analysis of extracts of various cell lines
Figure 1. Western blotting validation for Anti-ATP6V1A Antibody A10401 Western blot analysis of extracts of various cell lines

Anti-ATP6V1A Antibody

A10401
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP6V1A
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-ATP6V1A Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID523
  • Target name
    ATP6V1A
  • Target description
    ATPase H+ transporting V1 subunit A
  • Target synonyms
    ARCL2D, ATP6A1, ATP6V1A1, DEE93, HO68, IECEE3, VA68, VPP2, Vma1, V-type proton ATPase catalytic subunit A, ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A, ATPase, H+ transporting, lysosomal, subunit A1, H(+)-transporting two-sector ATPase, subunit A, H+-transporting ATPase chain A, vacuolar (VA68 type), V-ATPase 69 kDa subunit 1, V-ATPase A subunit 1, V-ATPase subunit A, V-type proton ATPase (V-ATPase) catalytic subunit A, vacuolar proton pump alpha subunit 1, vacuolar proton pump subunit alpha, vacuolar-type H(+)-ATPase
  • Host
    Rabbit
  • Protein IDP38606
  • Protein Name
    V-type proton ATPase catalytic subunit A
  • Scientific Description
    Boster Bio Anti-ATP6V1A Antibody catalog # A10401. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1A antigen affinity purified polyclonal antibody (Catalog # A10401-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP6V1A at approximately 70 kDa. The expected band size for ATP6V1A is at 70 kDa.
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