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Immunohistochemistry analysis in human cerebral cortex and cervix, uterine tissues using HPA008147 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and cervix, uterine tissues using HPA008147 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and cervix, uterine tissues using HPA008147 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.

Anti-ATP6V1B2 Antibody

HPA008147
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP6V1B2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ATP6V1B2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID526
  • Target name
    ATP6V1B2
  • Target description
    ATPase H+ transporting V1 subunit B2
  • Target synonyms
    ATP6B1B2, ATP6B2, DOOD, HO57, VATB, VPP3, Vma2, ZLS2, V-type proton ATPase subunit B, brain isoform, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2, H+ transporting two-sector ATPase, V-ATPase B2 subunit, V-ATPase subunit B 2, endomembrane proton pump 58 kDa subunit, testicular secretory protein Li 65, vacuolar H+-ATPase 56,000 subunit, vacuolar proton pump subunit B 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21281
  • Protein Name
    V-type proton ATPase subunit B, brain isoform
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ATP6V1B2 using anti-ATP6V1B2 antibody (A04927-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat H9C2(2-1) whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1B2 antigen affinity purified polyclonal antibody (Catalog # A04927-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP6V1B2 at approximately 57 kDa. The expected band size for ATP6V1B2 is at 57 kDa.
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