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Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.

Anti-ATP7A Antibody

HPA048107
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetATP7A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ATP7A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID538
  • Target name
    ATP7A
  • Target description
    ATPase copper transporting alpha
  • Target synonyms
    DSMAX, HMNX, MK, MNK, SMAX3, copper-transporting ATPase 1, ATPase, Cu++ transporting, alpha polypeptide, Cu++-transporting P-type ATPase, Menkes disease-associated protein, copper pump 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04656
  • Protein Name
    Copper-transporting ATPase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATP7A using anti-ATP7A antibody (A01085-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP7A antigen affinity purified polyclonal antibody (Catalog # A01085-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP7A at approximately 163 kDa. The expected band size for ATP7A is at 163 kDa.
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