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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Anti-ATXN1 Antibody

HPA008335
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetATXN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ATXN1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6310
  • Target name
    ATXN1
  • Target description
    ataxin 1
  • Target synonyms
    alternative ataxin1; ataxin-1; ATX1; D6S504E; SCA1; spinocerebellar ataxia type 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54253
  • Protein Name
    Ataxin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9422) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 1 at approximately 105 kDa. The expected band size for Ataxin 1 is at 105 kDa.
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