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Western Blot analysis of MOUSE-BRAIN cells using Ataxin-1 Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN cells using Ataxin-1 Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN cells using Ataxin-1 Polyclonal Antibody

ATXN1 Antibody

CSB-PA000943
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetATXN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATXN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6310
  • Target name
    ATXN1
  • Target description
    ataxin 1
  • Target synonyms
    ATX1, D6S504E, SCA1, ataxin-1, alternative ataxin1, spinocerebellar ataxia type 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54253
  • Protein Name
    Ataxin-1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9422) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 1 at approximately 105 kDa. The expected band size for Ataxin 1 is at 105 kDa.
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