Anti-B7-H4 [6H3]

Absolute Antibody

Anti-B7-H4 [6H3]

7

This antibody is specific for the IgC region of human B7-H4.

Humanized variants of this antibody 6H3V6 and 6H3V7 are available on request. This antibody is able to neutralize the effect of an B7-H4 blocking antibody (US9574000). Flow cytometry was performed on HEK293 cells that expressed full length human B7-H4 using this antibody (US9574000). This antibody induces robust internalization of B7-H4 expressed on the surface of both the B7-H4 transfected cell line and the B7-H4 positive breast cancer cell line SK-BR-3 (US9574000). Immunohistochemistry was performed on human tissue using this antibody (US9574000). An internalization assay was performed using this antibody on EG7 (B7-H4 negative cells), EG7.IG7 (B7-H4 low expressing cells), and EG7.IVB3 (B7-H4 high expressing cells) (US9574000). Antibody-Dependent Cell-Mediated Cytotoxicity assay was performed on EG7.hB7-H4 (clone IVB3) cells and SK-BR-3 cells using this antibody (US9574000). An ELISA was performed on B7-H4ECD his-tagged protein using this antibody (US9574000).

Functional Assay, Flow Cytometry, ELISA, ImmunoHistoChemistry

ELISA; FC; IHC; functional assay

Research Use Only

Monoclonal

6H3

VTCN1

V-set domain containing T cell activation inhibitor 1

B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7h.5; B7H4; B7-H4; B7S1; B7X; immune costimulatory protein B7-H4; PRO1291; T cell costimulatory molecule B7x; VCTN1; V-set domain-containing T-cell activation inhibitor 1

Mouse

IgG1

V-set domain-containing T-cell activation inhibitor 1

Human

Human

The original antibody was raised by immunizing a DO11.10 mouse with B7-H4.

-20°C,2°C to 8°C

12352203