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Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.

Anti-VTCN1 Antibody

HPA054200
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetVTCN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-VTCN1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79679
  • Target name
    VTCN1
  • Target description
    V-set domain containing T cell activation inhibitor 1
  • Target synonyms
    B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7h.5; B7H4; B7-H4; B7S1; B7X; immune costimulatory protein B7-H4; PRO1291; T cell costimulatory molecule B7x; VCTN1; V-set domain-containing T-cell activation inhibitor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z7D3
  • Protein Name
    V-set domain-containing T-cell activation inhibitor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VTCN1 using anti-VTCN1 antibody (A02821-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: moue liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VTCN1 antigen affinity purified polyclonal antibody (Catalog # A02821-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VTCN1 at approximately 75 kDa. The expected band size for VTCN1 is at 31 kDa.
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