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Anti-BA46 [Mc3]

Ab01178-1.1
Absolute Antibody
ApplicationsRadioImmunoAssay
Product group Antibodies
TargetMFGE8
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-BA46 [Mc3]
  • Delivery Days Customer
    7
  • Application Supplier Note
    In radioimmunoassays Mc3 bound to membranes of HMFG, MCF-7, MDA-MB- 157, SKBR-3, and BT-20 cells but not breast fibroblast, Hela, HT-29, Bristol 8, Hs294T, Hs695T, Hs785T, Hs769T, A498, PANC-1, MIA PaCa-2 or A-549 cells (Ceriani et al, 1983). Human Mc3 was characterised using biodistribution studies and shown to have similar properties to the murine Mc3 (Couto et al, 1995).
  • Applications
    RadioImmunoAssay
  • Applications Supplier
    Bio-distribution; RIA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Mc3
  • Gene ID4240
  • Target name
    MFGE8
  • Target description
    milk fat globule EGF and factor V/VIII domain containing
  • Target synonyms
    BA46; breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; lactadherin; medin; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ08431
  • Protein Name
    Lactadherin
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunising BALB/c mice with delipidated human milk fat globules.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human milk 5 microl, Lane 2: human milk 5 microl, Lane 3: human milk 2.5 microl, Lane 4: human milk 2.5 microl. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/MFGE8 antigen affinity purified polyclonal antibody (Catalog # A02518-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/MFGE8 at approximately 43 kDa. The expected band size for Milk Fat Globule 1/MFGE8 is at 43 kDa.
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