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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: MFGE8 antibody at 1.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 32, 38, 39 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: MFGE8 antibody at 1.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 32, 38, 39 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: MFGE8 antibody at 1.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 32, 38, 39 kDa Observed band size: 44 kDa

MFGE8 Antibody

CSB-PA07974A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMFGE8
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Overview

  • Supplier
    Cusabio
  • Product Name
    MFGE8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4240
  • Target name
    MFGE8
  • Target description
    milk fat globule EGF and factor V/VIII domain containing
  • Target synonyms
    BA46; breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; lactadherin; medin; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08431
  • Protein Name
    Lactadherin
  • Scientific Description
    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization By similarity. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Ref.15.Medin is the main constituent of aortic medial amyloid.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human milk 5 microl, Lane 2: human milk 5 microl, Lane 3: human milk 2.5 microl, Lane 4: human milk 2.5 microl. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/MFGE8 antigen affinity purified polyclonal antibody (Catalog # A02518-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/MFGE8 at approximately 43 kDa. The expected band size for Milk Fat Globule 1/MFGE8 is at 43 kDa.
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