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Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Anti-BAD Antibody

HPA028185
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBAD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BAD
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID572
  • Target name
    BAD
  • Target description
    BCL2 associated agonist of cell death
  • Target synonyms
    BBC2, BCL2L8, bcl2-associated agonist of cell death, BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-binding component 6, BCL2-binding protein, bcl-2-binding component 6, bcl-2-like protein 8, bcl-XL/Bcl-2-associated death promoter, bcl2 antagonist of cell death, bcl2-L-8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92934
  • Protein Name
    Bcl2-associated agonist of cell death
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of BAD using anti-BAD antibody (A03520-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse Raw264.7 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAD antigen affinity purified polyclonal antibody (Catalog # A03520-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAD at approximately 22 kDa. The expected band size for BAD is at 18 kDa.
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