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Western Blot analysis of Jurkat cells using Bad Polyclonal Antibody
Western Blot analysis of Jurkat cells using Bad Polyclonal Antibody
Western Blot analysis of Jurkat cells using Bad Polyclonal Antibody

BAD Antibody

CSB-PA000966
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBAD
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Overview

  • Supplier
    Cusabio
  • Product Name
    BAD Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID572
  • Target name
    BAD
  • Target description
    BCL2 associated agonist of cell death
  • Target synonyms
    BBC2; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; bcl2-associated agonist of cell death; bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; bcl2-L-8; bcl-2-like protein 8; BCL-X/BCL-2 binding protein; bcl-XL/Bcl-2-associated death promoter
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92934
  • Protein Name
    Bcl2-associated agonist of cell death
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bad ) Polyclonal Antibody, Unconjugated (bs-1304R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Figure 1. Western blot analysis of BAD using anti-BAD antibody (A03520-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse Raw264.7 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAD antigen affinity purified polyclonal antibody (Catalog # A03520-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAD at approximately 22 kDa. The expected band size for BAD is at 18 kDa.
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