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Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity.
Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity.
Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity.

Anti-BAX Antibody

HPA027878
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBAX
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BAX Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID581
  • Target name
    BAX
  • Target description
    BCL2 associated X, apoptosis regulator
  • Target synonyms
    BCL2L4, apoptosis regulator BAX, BCL2 associated X protein, BCL2-associated X protein omega, Baxdelta2(G8)-RFS protein, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, bcl-2-like protein 4, bcl2-L-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07812
  • Protein Name
    Apoptosis regulator BAX
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –BAX Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
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Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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