
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –BAX Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
Bax Polyclonal Antibody
BS-0127R
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBAX
Overview
- SupplierBioss
- Product NameBax Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsBCL2L4, apoptosis regulator BAX, BCL2 associated X protein, BCL2-associated X protein omega, Baxdelta2(G8)-RFS protein, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, bcl-2-like protein 4, bcl2-L-4
- HostRabbit
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- Storage Instruction-20°C
- UNSPSC12352203
References
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