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Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Anti-BBS1 Antibody

HPA058283
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBBS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BBS1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID582
  • Target name
    BBS1
  • Target description
    Bardet-Biedl syndrome 1
  • Target synonyms
    BBS2L2, BBSome complex member BBS1, BBS2-like protein 2, bardet-Biedl syndrome 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NFJ9
  • Protein Name
    BBSome complex member BBS1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BBS1 using anti-BBS1 antibody (A03072-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS1 antigen affinity purified polyclonal antibody (Catalog # A03072-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BBS1 at approximately 65 kDa. The expected band size for BBS1 is at 65 kDa.
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