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Immunohistochemical staining of human kidney shows strong granular positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular positivity in cells in tubules.

Anti-BCKDHA Antibody

HPA036640
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBCKDHA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BCKDHA
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID593
  • Target name
    BCKDHA
  • Target description
    branched chain keto acid dehydrogenase E1 subunit alpha
  • Target synonyms
    BCKDE1A, MSU, MSUD1, MSUD1A, OVD1A, 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, 2-oxoisovalerate dehydrogenase (lipoamide), BCKDH E1-alpha, branched chain keto acid dehydrogenase E1 alpha protein, branched chain keto acid dehydrogenase E1, alpha polypeptide, branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12694
  • Protein Name
    2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of BCKDHA using anti-BCKDHA antibody (A04561-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCKDHA antigen affinity purified polyclonal antibody (Catalog # A04561-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCKDHA at approximately 50 kDa. The expected band size for BCKDHA is at 50 kDa.
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