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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: BCKDHA antibody at 4.58microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: BCKDHA antibody at 4.58microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: BCKDHA antibody at 4.58microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa

BCKDHA Antibody

CSB-PA002605LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBCKDHA
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Overview

  • Supplier
    Cusabio
  • Product Name
    BCKDHA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID593
  • Target name
    BCKDHA
  • Target description
    branched chain keto acid dehydrogenase E1 subunit alpha
  • Target synonyms
    2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1-alpha; branched chain keto acid dehydrogenase E1 alpha protein; branched chain keto acid dehydrogenase E1, alpha polypeptide; branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; MSU; MSUD1; OVD1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12694
  • Protein Name
    2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
  • Scientific Description
    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BCKDHA using anti-BCKDHA antibody (A04561-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCKDHA antigen affinity purified polyclonal antibody (Catalog # A04561-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCKDHA at approximately 50 kDa. The expected band size for BCKDHA is at 50 kDa.
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