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Immunohistochemical staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.

Anti-BCKDK Antibody

HPA017995
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCKDK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BCKDK
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10295
  • Target name
    BCKDK
  • Target description
    branched chain keto acid dehydrogenase kinase
  • Target synonyms
    BCKDKD, BDK, branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDH kinase, BCKDHKIN, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14874
  • Protein Name
    [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of BCKDK using anti-BCKDK antibody (A07798-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCKDK antigen affinity purified polyclonal antibody (Catalog # A07798-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCKDK at approximately 46 kDa. The expected band size for BCKDK is at 46 kDa.
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