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BCKDK Antibody

LS-C409720
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCKDK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCKDK Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 37kDa/41kDa/46kDa, while the observed MW by Western blot was 46kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 37kDa/41kDa/46kDa, while the observed MW by Western blot was 46kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10295
  • Target name
    BCKDK
  • Target description
    branched chain keto acid dehydrogenase kinase
  • Target synonyms
    BCKDKD, BDK, branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDH kinase, BCKDHKIN, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BCKDK using anti-BCKDK antibody (A07798-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCKDK antigen affinity purified polyclonal antibody (Catalog # A07798-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCKDK at approximately 46 kDa. The expected band size for BCKDK is at 46 kDa.
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