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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, cytosol, midbody & vesicles.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, cytosol, midbody & vesicles.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, cytosol, midbody & vesicles.

Anti-BCL3 Antibody

HPA047514
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetBCL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BCL3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID602
  • Target name
    BCL3
  • Target description
    BCL3 transcription coactivator
  • Target synonyms
    BCL4, D19S37, B-cell lymphoma 3 protein, B cell CLL/lymphoma 3, B-cell leukemia/lymphoma 3, B-cell lymphoma 3-encoded protein, BCL-3, chronic lymphatic leukemia protein, proto-oncogene BCL3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20749
  • Protein Name
    B-cell lymphoma 3 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of BCL3 using anti-BCL3 antibody (A02773-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse Neuro-2a whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL3 antigen affinity purified polyclonal antibody (Catalog # A02773-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL3 at approximately 60KD. The expected band size for BCL3 is at 60KD.
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