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BCL3 antibody

10R-1639
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    BCL3 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCL3 using anti-BCL3 antibody (A02773-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse Neuro-2a whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL3 antigen affinity purified polyclonal antibody (Catalog # A02773-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL3 at approximately 60KD. The expected band size for BCL3 is at 60KD.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA
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RayBiotech
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Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti-BCL3 Polyclonal Antibody, Unconjugated (bs-6591R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD. Observed band size: 50kD
Product group Antibodies
Bioss
Bcl3 Polyclonal AntibodyBS-6591R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetBCL3
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Western Blot Positive WB detected in: Mouse heart tissue All lanes: BCL3 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 kDa Observed band size: 60 kDa
Product group Antibodies
Cusabio
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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, cytosol, midbody & vesicles.
Product group Antibodies
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Anti-BCL3 AntibodyA38798
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WB analysis of 293 cell lysate using GTX31868 BCL3 antibody. Working concentration : 1 μg/ml
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GeneTex
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