Anti-BCMA [CA8]

Absolute Antibody

Anti-BCMA [CA8]

9

The binding characterization of this antibody towards human and cynomolgus monkey recombinant BCMA was done using ELISA (US9273141). The binding of the antibody to membrane bound BCMA expressed on HEK293, H929, OPM-2, JJN-3 and U266 cells was done using flow cytometry (US9273141). This antibody neutralizes binding of both BCMA ligands BAFF and APRIL, and subsequently blocks the induction of NF-kappaB signaling cascades (PMID: 26370838). The EC50 values for chimeric CA8 were calculated to be 0.695 microg/mL and 0.773 microg/mL respectively (US9273141). This antibody neutralized both BAFF and APRIL induced NfkappaB cell signaling in H-929 cells. This antibody was also reported to kill BCMA expressing ARH77 cells via ADCC. This antibody was also used in the generation of an antibody-drug conjugate for possible application in the treatment of multiple myeloma (US9273141).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

CA8

CENPE

centromere protein E

CENP-E, KIF10, MCPH13, PPP1R61, centromere-associated protein E, Centromere autoantigen E (312kD), centromere protein E, 312kDa, kinesin family member 10, kinesin-7, kinesin-related protein CENPE, protein phosphatase 1, regulatory subunit 61

Mouse

IgG1

Centromere-associated protein E

-20°C,2°C to 8°C

12352203