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Immunohistochemical staining of human cerebral cortex shows strong positivity in neuronal processes.
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuronal processes.
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuronal processes.

Anti-BDKRB2 Antibody

HPA050841
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBDKRB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BDKRB2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID624
  • Target name
    BDKRB2
  • Target description
    bradykinin receptor B2
  • Target synonyms
    B2R, BK-2, BK2, BKR2, BRB2, B2 bradykinin receptor, BK-2 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30411
  • Protein Name
    B2 bradykinin receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB10047). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates, Lane 3: MCF-7 whole cell lysates. Lane 4: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (Catalog # PB10047) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDKRB2 at approximately 50 kDa. The expected band size for BDKRB2 is at 44 kDa.
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