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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: BDKRB2 antibody at 4.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 42 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: BDKRB2 antibody at 4.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 42 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: BDKRB2 antibody at 4.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 42 kDa Observed band size: 45 kDa

BDKRB2 Antibody

CSB-PA002652LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBDKRB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    BDKRB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID624
  • Target name
    BDKRB2
  • Target description
    bradykinin receptor B2
  • Target synonyms
    B2R, BK-2, BK2, BKR2, BRB2, B2 bradykinin receptor, BK-2 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30411
  • Protein Name
    B2 bradykinin receptor
  • Scientific Description
    Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB10047). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates, Lane 3: MCF-7 whole cell lysates. Lane 4: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (Catalog # PB10047) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDKRB2 at approximately 50 kDa. The expected band size for BDKRB2 is at 44 kDa.
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