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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus tubules and leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus tubules and leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus tubules and leydig cells.

Anti-BEX3 Antibody

HPA018886
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetBEX3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BEX3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27018
  • Target name
    BEX3
  • Target description
    brain expressed X-linked 3
  • Target synonyms
    Bex; brain-expressed X-linked protein 3; DXS6984E; HGR74; NADE; nerve growth factor receptor (TNFRSF16) associated protein 1; NGFRAP1; ovarian granulosa cell 13.0 kDa protein HGR74; p75NTR-associated cell death executor; protein BEX3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00994
  • Protein Name
    Protein BEX3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BEX3 using anti-BEX3 antibody (A09675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BEX3 antigen affinity purified polyclonal antibody (Catalog # A09675-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BEX3 at approximately 28 kDa. The expected band size for BEX3 is at 28 kDa.
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