Western blot using anti-Bmf (mouse/rat), mAb (17A9) (Prod. No. AG-20T-0131) detecting endogenous Bmf in mouse spleen, lymph node and thymus as two bands of ~25 and ~30 kDa, but not in tissues from two knock-out Bmf strains del 372 and del 376.
Western blot using anti-Bmf (mouse/rat), mAb (17A9) (Prod. No. AG-20T-0131) detecting endogenous Bmf in mouse spleen, lymph node and thymus as two bands of ~25 and ~30 kDa, but not in tissues from two knock-out Bmf strains del 372 and del 376.
anti-Bmf (mouse/rat), mAb (17A9)
AG-20T-0131
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetBmf
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Bmf (mouse/rat), mAb (17A9)
- Delivery Days Customer10
- Antibody SpecificityRecognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. Does not cross-react with human Bmf.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID17A9
- Concentration1 mg/ml
- Estimated Purity>95%
- FormulationLiquid
- Gene ID171543
- Target nameBmf
- Target descriptionBCL2 modifying factor
- Target synonymsAW260063; AW411872; bcl-2-modifying factor
- HostRat
- IsotypeIgG2a
- Protein IDQ91ZE9
- Protein NameBcl-2-modifying factor
- Scientific DescriptionBmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis. - Monoclonal Antibody. Recognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. Does not cross-react with human Bmf. Isotype: Rat IgG2akappa. Clone: 17A9. Applications: ELISA, FACS, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203