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Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.

Anti-BMI1 Antibody

HPA030471
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetBMI1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BMI1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID648
  • Target name
    BMI1
  • Target description
    BMI1 proto-oncogene, polycomb ring finger
  • Target synonyms
    FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1, polycomb complex protein BMI-1, B lymphoma Mo-MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, BMI1 polycomb ring finger proto-oncogene, murine leukemia viral (bmi-1) oncogene homolog, polycomb group RING finger protein 4, polycomb group protein Bmi1, ring finger protein 51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35226
  • Protein Name
    Polycomb complex protein BMI-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of BMI1 using anti-BMI1 antibody (PB9133). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2O whole cell lysates Lane 2: human PC-3 whole cell lysates Lane 3: human HEK293 whole cell lysates Lane 4: human Caco-2 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (Catalog # PB9133) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 43-45KD. The expected band size for BMI1 is at 39KD.
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