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BMI1 Polyclonal Antibody

CAC14038
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBMI1
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Overview

  • Supplier
    Biomatik
  • Product Name
    BMI1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID648
  • Target name
    BMI1
  • Target description
    BMI1 proto-oncogene, polycomb ring finger
  • Target synonyms
    FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1, polycomb complex protein BMI-1, B lymphoma Mo-MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, BMI1 polycomb ring finger proto-oncogene, murine leukemia viral (bmi-1) oncogene homolog, polycomb group RING finger protein 4, polycomb group protein Bmi1, ring finger protein 51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35226
  • Protein Name
    Polycomb complex protein BMI-1
  • Scientific Description
    The BMI1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMI1 using anti-BMI1 antibody (PB9133). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2O whole cell lysates Lane 2: human PC-3 whole cell lysates Lane 3: human HEK293 whole cell lysates Lane 4: human Caco-2 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (Catalog # PB9133) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 43-45KD. The expected band size for BMI1 is at 39KD.
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