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Anti-BPIFA1 Antibody

144-08634
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetBPIFA1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-BPIFA1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51297
  • Target name
    BPIFA1
  • Target description
    BPI fold containing family A member 1
  • Target synonyms
    LUNX, NASG, PLUNC, SPLUNC1, SPURT, bA49G10.5, BPI fold-containing family A member 1, ligand-binding protein RYA3, lung-specific protein X, nasopharyngeal carcinoma-related protein, palate lung and nasal epithelium clone protein, palate, lung and nasal epithelium associated, protein Plunc, secretory protein in upper respiratory tracts, short PLUNC1, tracheal epithelium enriched protein, von Ebner protein Hl
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP55
  • Protein Name
    BPI fold-containing family A member 1
  • Scientific Description
    BPIFA1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Plunc/BPIFA1 using anti-Plunc/BPIFA1 antibody (A03162-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plunc/BPIFA1 antigen affinity purified polyclonal antibody (Catalog # A03162-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plunc/BPIFA1 at approximately 27 kDa. The expected band size for Plunc/BPIFA1 is at 27 kDa.
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