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Anti-BRD3 [RAB-C280]

Ab01743-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
TargetBRD3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-BRD3 [RAB-C280]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: IP-MS; IF.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation
  • Applications Supplier
    IP-MS; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C280
  • Gene ID8019
  • Target name
    BRD3
  • Target description
    bromodomain containing 3
  • Target synonyms
    FSHRG2, ORFX, RING3L, bromodomain-containing protein 3, RING3-like protein, female sterile homeotic related gene 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15059
  • Protein Name
    Bromodomain-containing protein 3
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing BRD3 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BRD3 using anti-BRD3 antibody (A04044-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD3 antigen affinity purified polyclonal antibody (Catalog # A04044-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD3 at approximately 80 kDa. The expected band size for BRD3 is at 80 kDa.
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