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Western Blot analysis of A549 cells using BRD3 Polyclonal Antibody
Western Blot analysis of A549 cells using BRD3 Polyclonal Antibody
Western Blot analysis of A549 cells using BRD3 Polyclonal Antibody

BRD3 Antibody

CSB-PA001045
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetBRD3
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Overview

  • Supplier
    Cusabio
  • Product Name
    BRD3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8019
  • Target name
    BRD3
  • Target description
    bromodomain containing 3
  • Target synonyms
    FSHRG2, ORFX, RING3L, bromodomain-containing protein 3, RING3-like protein, female sterile homeotic related gene 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15059
  • Protein Name
    Bromodomain-containing protein 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BRD3 using anti-BRD3 antibody (A04044-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD3 antigen affinity purified polyclonal antibody (Catalog # A04044-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD3 at approximately 80 kDa. The expected band size for BRD3 is at 80 kDa.
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