anti-BRG1 antibody

Arigo Biolaboratories

anti-BRG1 antibody

23

ImmunoPrecipitation, Western Blot

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

SMARCA4

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4

ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; BRG1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; mitotic growth and transcription activator; MRD16; nuclear protein GRB1; protein brahma homolog 1; protein BRG-1; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; SNF2-like 4; sucrose nonfermenting-like 4; SWI2; transcription activator BRG1

Mouse

IgG1

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation.Component of the CREST-BRG1 complex,a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex.In resting neurons,transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex.Upon calcium influx,RB1 is dephosphorylated by calcineurin,which leads to release of the repressor complex.At the same time,there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism,which leads to transcriptional activation.The CREST-BRG1 complex also binds to the NR2B promoter,and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex).During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF).The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells.The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals,while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues By similarity.Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex,a chromatin-remodeling complex recruited by vitamin D receptor (VDR),which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.

Human

-20°C

12352203