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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Anti-BRSK1 Antibody

HPA061719
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBRSK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BRSK1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84446
  • Target name
    BRSK1
  • Target description
    BR serine/threonine kinase 1
  • Target synonyms
    SAD-B, hSAD1, serine/threonine-protein kinase BRSK1, BR serine/threonine-protein kinase 1, SAD1 homolog, SAD1 kinase, brain-selective kinase 1, brain-specific serine/threonine-protein kinase 1, protein kinase SAD1A, serine/threonine-protein kinase SAD-B, synapses of Amphids Defective homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDC3
  • Protein Name
    Serine/threonine-protein kinase BRSK1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of BRSK1 using anti-BRSK1 antibody (A05997-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRSK1 antigen affinity purified polyclonal antibody (Catalog # A05997-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRSK1 at approximately 90 kDa. The expected band size for BRSK1 is at 90 kDa.
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