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Immunohistochemical staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Anti-BST2 Antibody

HPA017060
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetBST2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BST2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID684
  • Target name
    BST2
  • Target description
    bone marrow stromal cell antigen 2
  • Target synonyms
    antiviral factor tetherin; bone marrow stromal antigen 2; BST-2; CD317; HM1.24; HM1.24 antigen; NPC-A-7; TETHERIN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ10589
  • Protein Name
    Bone marrow stromal antigen 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BST2/Tetherin using anti-BST2/Tetherin antibody (A00934-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BST2/Tetherin antigen affinity purified polyclonal antibody (Catalog # A00934-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BST2/Tetherin at approximately 25-45 kDa. The expected band size for BST2/Tetherin is at 20 kDa.
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Lane 1: Hela Cells; Lane 2: SiHa Cells; Probed with BST2 (1C3) Monoclonal Antibody (bsm-54196R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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