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Western Blot analysis of SKOV3 cells using BST-2 Polyclonal Antibody
Western Blot analysis of SKOV3 cells using BST-2 Polyclonal Antibody
Western Blot analysis of SKOV3 cells using BST-2 Polyclonal Antibody

BST2 Antibody

CSB-PA006272
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBST2
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Overview

  • Supplier
    Cusabio
  • Product Name
    BST2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID684
  • Target name
    BST2
  • Target description
    bone marrow stromal cell antigen 2
  • Target synonyms
    antiviral factor tetherin; bone marrow stromal antigen 2; BST-2; CD317; HM1.24; HM1.24 antigen; NPC-A-7; TETHERIN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ10589
  • Protein Name
    Bone marrow stromal antigen 2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BST2/Tetherin using anti-BST2/Tetherin antibody (A00934-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BST2/Tetherin antigen affinity purified polyclonal antibody (Catalog # A00934-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BST2/Tetherin at approximately 25-45 kDa. The expected band size for BST2/Tetherin is at 20 kDa.
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