
Figure 1. Western blot analysis of BTG2 using anti-BTG2 antibody (A01461-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTG2 antigen affinity purified polyclonal antibody (Catalog # A01461-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTG2 at approximately 22 kDa. The expected band size for BTG2 is at 17 kDa.
Anti-BTG2 Antibody Picoband(r)
A01461-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetBTG2
Overview
- SupplierBoster Bio
- Product NameAnti-BTG2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID7832
- Target nameBTG2
- Target descriptionBTG anti-proliferation factor 2
- Target synonymsAPRO1; B-cell translocation gene 2; BTG family member 2; nerve growth factor-inducible anti-proliferative; NGF-inducible anti-proliferative protein PC3; PC3; pheochromacytoma cell-3; protein BTG2; TIS21
- HostRabbit
- IsotypeIgG
- Protein IDP78543
- Protein NameProtein BTG2
- Scientific DescriptionBoster Bio Anti-BTG2 Antibody Picoband® catalog # A01461-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203