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Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Human liver cancer tissue, Primary antibody: CSB-PA124546(BTG2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Human liver cancer tissue, Primary antibody: CSB-PA124546(BTG2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Human liver cancer tissue, Primary antibody: CSB-PA124546(BTG2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes

BTG2 Antibody

CSB-PA124546
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBTG2
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Overview

  • Supplier
    Cusabio
  • Product Name
    BTG2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7832
  • Target name
    BTG2
  • Target description
    BTG anti-proliferation factor 2
  • Target synonyms
    APRO1; B-cell translocation gene 2; BTG family member 2; nerve growth factor-inducible anti-proliferative; NGF-inducible anti-proliferative protein PC3; PC3; pheochromacytoma cell-3; protein BTG2; TIS21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78543
  • Protein Name
    Protein BTG2
  • Scientific Description
    The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein is involved in the regulation of the G1/S transition of the cell cycle. Protein BTG2 also known as BTG family member 2 or NGF-inducible anti-proliferative protein PC3 or NGF-inducible protein TIS21, is a protein that in humans is encoded by the BTG2 gene (B-cell translocation gene 2) and in other mammals by the homologous Btg2 gene. This protein controls cell cycle progression and proneural genes expression by acting as a transcription coregulator that enhances or inhibits the activity of transcription factors.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BTG2 using anti-BTG2 antibody (A01461-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTG2 antigen affinity purified polyclonal antibody (Catalog # A01461-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTG2 at approximately 22 kDa. The expected band size for BTG2 is at 17 kDa.
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