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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Anti-CA10 Antibody

HPA054825
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCA10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CA10 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56934
  • Target name
    CA10
  • Target description
    carbonic anhydrase 10
  • Target synonyms
    CA-RPX, CARPX, HUCEP-15, carbonic anhydrase-related protein 10, CA-RP X, CARP X, carbonic anhydrase X, carbonic anhydrase-related protein X, cerebral protein-15, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS85
  • Protein Name
    Carbonic anhydrase-related protein 10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Carbonic anhydrase 10/CA10 using anti-Carbonic anhydrase 10/CA10 antibody (A07237-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic anhydrase 10/CA10 antigen affinity purified polyclonal antibody (Catalog # A07237-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic anhydrase 10/CA10 at approximately 38 kDa. The expected band size for Carbonic anhydrase 10/CA10 is at 38 kDa.
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