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ProductsBack/CA10 / Carbonic Andhydrase X Antibody (FITC)

CA10 / Carbonic Andhydrase X Antibody (FITC)

LS-C677352
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetCA10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CA10 / Carbonic Andhydrase X Antibody (FITC)
  • Delivery Days Customer
    14
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID56934
  • Target name
    CA10
  • Target description
    carbonic anhydrase 10
  • Target synonyms
    CA-RPX, CARPX, HUCEP-15, carbonic anhydrase-related protein 10, CA-RP X, CARP X, carbonic anhydrase X, carbonic anhydrase-related protein X, cerebral protein-15, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Carbonic anhydrase 10/CA10 using anti-Carbonic anhydrase 10/CA10 antibody (A07237-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic anhydrase 10/CA10 antigen affinity purified polyclonal antibody (Catalog # A07237-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic anhydrase 10/CA10 at approximately 38 kDa. The expected band size for Carbonic anhydrase 10/CA10 is at 38 kDa.
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