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Anti-CACNA1A (Center) Antibody

102-24765
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetCACNA1A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CACNA1A (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB53551
  • Gene ID773
  • Target name
    CACNA1A
  • Target description
    calcium voltage-gated channel subunit alpha1 A
  • Target synonyms
    APCA, BI, CACNL1A4, CAV2.1, DEE42, EA2, EIEE42, FHM, HPCA, MHP, MHP1, SCA6, voltage-dependent P/Q-type calcium channel subunit alpha-1A, brain calcium channel 1, brain calcium channel I, calcium channel, L type, alpha-1 polypeptide, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, fetal brain Ca2+ voltage-gated channel alpha1A pore-forming subunit, voltage-gated calcium channel subunit alpha Cav2.1
  • Host
    Rabbit
  • Protein IDO00555
  • Protein Name
    Voltage-dependent P/Q-type calcium channel subunit alpha-1A
  • Scientific Description
    Rabbit Anti-CACNA1A (Center) Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CACNA1A using anti-CACNA1A antibody (A00647). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA1A antigen affinity purified polyclonal antibody (A00647) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACNA1A at approximately 300 kDa. The expected band size for CACNA1A is at 282 kDa.
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