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Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CAF1B CHAF1B Antibody

A30479
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHAF1B
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CAF1B CHAF1B Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID8208
  • Target name
    CHAF1B
  • Target description
    chromatin assembly factor 1 subunit B
  • Target synonyms
    CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7, chromatin assembly factor 1 subunit B, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, M-phase phosphoprotein 7, chromatin assembly factor I p60 subunit, human chromatin assembly factor-I p60 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13112
  • Protein Name
    Chromatin assembly factor 1 subunit B
  • Scientific Description
    Boster Bio Anti-CAF1B CHAF1B Antibody (Catalog# A30479). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p60 CAF1 using anti-p60 CAF1 antibody (M03959-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p60 CAF1 antigen affinity purified monoclonal antibody (Catalog # M03959-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p60 CAF1 at approximately 70 kDa. The expected band size for p60 CAF1 is at 61 kDa.
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