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Anti-Calponin protein [24H5]

AB03312-10.0-BT
Absolute Antibody
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCNN2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Calponin protein [24H5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody specifically detected Calponin beta protein in Western blot analysis. The antibody 24H5 and the commercial antibody CALP were used to stain uterine fibroids and liver tissue. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 24H5 was higher than the commercial antibody (CN113234155A).
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    24H5
  • Gene ID1265
  • Target name
    CNN2
  • Target description
    calponin 2
  • Target synonyms
    calponin H2, smooth muscle; calponin-2; neutral calponin
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ99439
  • Protein Name
    Calponin-2
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calponin 2/CNN2 using anti-Calponin 2/CNN2 antibody (A08680-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 2/CNN2 antigen affinity purified polyclonal antibody (Catalog # A08680-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 2/CNN2 at approximately 34 kDa. The expected band size for Calponin 2/CNN2 is at 34 kDa.
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