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Western Blot analysis of COLO205 cells using Calponin 2 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Calponin 2 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Calponin 2 Polyclonal Antibody

CNN2 Antibody

CSB-PA001190
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetCNN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CNN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1265
  • Target name
    CNN2
  • Target description
    calponin 2
  • Target synonyms
    calponin H2, smooth muscle; calponin-2; neutral calponin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99439
  • Protein Name
    Calponin-2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calponin 2/CNN2 using anti-Calponin 2/CNN2 antibody (A08680-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 2/CNN2 antigen affinity purified polyclonal antibody (Catalog # A08680-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 2/CNN2 at approximately 34 kDa. The expected band size for Calponin 2/CNN2 is at 34 kDa.
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