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Immunohistochemical staining of human Cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.
Immunohistochemical staining of human Cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.
Immunohistochemical staining of human Cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.

Anti-CAMK2A Antibody

HPA051783
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCAMK2A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CAMK2A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID815
  • Target name
    CAMK2A
  • Target description
    calcium/calmodulin dependent protein kinase II alpha
  • Target synonyms
    CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63, calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, caM kinase II subunit alpha, caMK-II subunit alpha, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase type II alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQM7
  • Protein Name
    Calcium/calmodulin-dependent protein kinase type II subunit alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CaMKII alpha using anti-CaMKII alpha antibody (A03241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII alpha antigen affinity purified polyclonal antibody (Catalog # A03241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII alpha at approximately 50, 60KD. The expected band size for CaMKII alpha is at 54KD.
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