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ProductsBack/CAMK2A + CAMK2B + CAMK2D Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CaMK2 Polyclonal Antibody, Unconjugated (bs-0541R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CaMK2 Polyclonal Antibody, Unconjugated (bs-0541R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CaMK2 Polyclonal Antibody, Unconjugated (bs-0541R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.

CAMK2A + CAMK2B + CAMK2D Polyclonal Antibody

BS-0541R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCAMK2A
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Overview

  • Supplier
    Bioss
  • Product Name
    CAMK2A + CAMK2B + CAMK2D Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID815
  • Target name
    CAMK2A
  • Target description
    calcium/calmodulin dependent protein kinase II alpha
  • Target synonyms
    CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63, calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, caM kinase II subunit alpha, caMK-II subunit alpha, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase type II alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li W, Gao H, Gao J, et al. Upregulation of MMP-9 and CaMKII prompts cardiac electrophysiological changes that predispose denervated transplanted hearts to arrhythmogenesis after prolonged cold ischemic storage. Biomed Pharmacother. 2019,112:108641. doi: 10.1016/j.biopha.2019.108641
    Read this paper

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Figure 1. Western blot analysis of CaMKII alpha using anti-CaMKII alpha antibody (A03241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII alpha antigen affinity purified polyclonal antibody (Catalog # A03241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII alpha at approximately 50, 60KD. The expected band size for CaMKII alpha is at 54KD.
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