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Anti-Carbonic Anhydrase IX Antibody

Anti-Carbonic Anhydrase IX Antibody

A34165
Antibodies.com
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Carbonic Anhydrase IX Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1.0 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Scientific Description
    Mouse monoclonal antibody to Carbonic Anhydrase IX
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.
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