
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Carbonic Anhydrase IX antibody
GTX128428
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCA9
Overview
- SupplierGeneTex
- Product NameCarbonic Anhydrase IX antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.12 mg/ml
- ConjugateUnconjugated
- Gene ID768
- Target nameCA9
- Target descriptioncarbonic anhydrase 9
- Target synonymsCAIX, MN, carbonic anhydrase 9, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase IX, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250
- HostRabbit
- IsotypeIgG
- Protein IDQ16790
- Protein NameCarbonic anhydrase 9
- Scientific DescriptionCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
















![Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636876/GTX636876_T-44620_20220826_ICC_IF_22083119_865.webp)