More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Carbonic Anhydrase IX antibody
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody (GTX128428) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Carbonic Anhydrase IX antibody

GTX128428
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCA9
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Carbonic Anhydrase IX antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.97 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID768
  • Target name
    CA9
  • Target description
    carbonic anhydrase 9
  • Target synonyms
    CAIX, MN, carbonic anhydrase 9, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase IX, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16790
  • Protein Name
    Carbonic anhydrase 9
  • Scientific Description
    Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kang HS, Kwon HY, Kim IK, et al. Intermittent hypoxia exacerbates tumor progression in a mouse model of lung cancer. Sci Rep. 2020,10(1):1854. doi: 10.1038/s41598-020-58906-7
    Read this paper
  • Han Y, Zhou X, Qian Y, et al. Hypoxia-targeting dendritic MRI contrast agent based on internally hydroxy dendrimer for tumor imaging. Biomaterials. 2019,213:119195. doi: 10.1016/j.biomaterials.2019.05.006
    Read this paper
  • Guo X, Zheng H, Luo W, et al. 5T4-specific chimeric antigen receptor modification promotes the immune efficacy of cytokine-induced killer cells against nasopharyngeal carcinoma stem cell-like cells. Sci Rep. 2017,7(1):4859. doi: 10.1038/s41598-017-04756-9
    Read this paper
  • Hua KT, Liu YF, Hsu CL, et al. 3'UTR polymorphisms of carbonic anhydrase IX determine the miR-34a targeting efficiency and prognosis of hepatocellular carcinoma. Sci Rep. 2017,7(1):4466. doi: 10.1038/s41598-017-04732-3
    Read this paper
  • Yeh YH, Wang SW, Yeh YC, et al. Rhapontigenin inhibits TGF-β-mediated epithelial‑mesenchymal transition via the PI3K/AKT/mTOR pathway and is not associated with HIF-1α degradation. Oncol Rep. 2016,35(5):2887-95. doi: 10.3892/or.2016.4664
    Read this paper
  • Valtorta S, Belloli S, Sanvito F, et al. Comparison of 18F-fluoroazomycin-arabinofuranoside and 64Cu-diacetyl-bis(N4-methylthiosemicarbazone) in preclinical models of cancer. J Nucl Med. 2013,54(7):1106-12. doi: 10.2967/jnumed.112.111120
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Human Carbonic Anhydrase IX [M75]Ab00414-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
ReactivityHuman
TargetCA9
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CA9 Antibody144-01658
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCA9
  • SizePrice
Product group Antibodies
Biosynth
CA9 antibody10R-3570
  • SizePrice
Figure 1. Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband(r)A01083-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCA9
  • SizePrice
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract (untreated) B. 30 μg HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 24 hr) C. 30u g HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 48 hr) D. 30 μg HeLa whole cell lysate/extract (untreated) E. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24 hr) F. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 48 hr) 7.5 % SDS-PAGE Carbonic Anhydrase IX antibody [GT14212] (GTX629747) dilution: 1:1000
Product group Antibodies
GeneTex
Carbonic Anhydrase IX antibody [GT14212]GTX629747
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCA9
  • SizePrice
Carbonic Anhydrase IX antibody [GT546] detects Carbonic Anhydrase IX protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract (untreated) B. 30 μg HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 48 hr) 7.5 % SDS-PAGE Carbonic Anhydrase IX antibody [GT546] (GTX629820) dilution: 1:1000
Product group Antibodies
GeneTex
Carbonic Anhydrase IX antibody [GT546]GTX629820
ApplicationsWestern Blot
ReactivityHuman
TargetCA9
  • SizePrice
Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.
Product group Antibodies
GeneTex
Carbonic Anhydrase IX antibody [HL1410]GTX636876
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCA9
  • SizePrice
Product group Antibodies
Biomatik
Carbonic Anhydrase IX (CA9) Polyclonal AntibodyCAU23602
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityPorcine
TargetCA9
  • SizePrice
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
Product group Antibodies
Cusabio
CA9 Monoclonal AntibodyCSB-MA000234
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
TargetCA9
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CA9 / Carbonic Anhydrase IX Antibody (clone 9B5)LS-C765000
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCA9
  • SizePrice