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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Anti-CASP1 Antibody

HPA003056
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CASP1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID834
  • Target name
    CASP1
  • Target description
    caspase 1
  • Target synonyms
    ICE, IL1BC, P45, caspase-1, IL-1 beta-converting enzyme, IL1B-convertase, caspase 1, apoptosis-related cysteine peptidase, interleukin 1, beta, convertase, interleukin 1-B converting enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29466
  • Protein Name
    Caspase-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Caspase 1(P20)/CASP1 using anti-Caspase 1(P20)/CASP1 antibody (A00048-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-2 whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 1(P20)/CASP1 antigen affinity purified polyclonal antibody (Catalog # A00048-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 1(P20)/CASP1 at approximately 47 kDa. The expected band size for Caspase 1(P20)/CASP1 is at 47 kDa.
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