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MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Caspase-1 P10 Polyclonal Antibody

BS-0169R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCASP1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Caspase-1 P10 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID834
  • Target name
    CASP1
  • Target description
    caspase 1
  • Target synonyms
    CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase; caspase-1; caspase-1 isoform alpha; ICE; IL-1 beta-converting enzyme; IL1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; P45
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29466
  • Protein Name
    Caspase-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Estrogen deficiency aggravates apical periodontitis by regulating NLRP3/caspase-1/IL-1beta axis. Guan X et al., 2020, Am J Transl Res
    Read more
  • Curcumin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation in mice. Chen Y et al., 2019 Oct, Biomed Pharmacother
    Read more
  • Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes. Liao J et al., 2019 Jun 15, Ecotoxicol Environ Saf
    Read more
  • Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes. Daverey A et al., 2016 Oct 1, Neuroscience
    Read more
  • Estrogen-provided cardiac protection following burn trauma is mediated through a reduction in mitochondria-derived DAMPs. Yao X et al., 2014 Mar, Am J Physiol Heart Circ Physiol
    Read more
  • Drug-induced apoptosis of Echinococcus granulosus protoscoleces. Hu H et al., 2011 Aug, Parasitol Res
    Read more

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Figure 1. Western blot analysis of Caspase 1(P20)/CASP1 using anti-Caspase 1(P20)/CASP1 antibody (A00048-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-2 whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 1(P20)/CASP1 antigen affinity purified polyclonal antibody (Catalog # A00048-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 1(P20)/CASP1 at approximately 47 kDa. The expected band size for Caspase 1(P20)/CASP1 is at 47 kDa.
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