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Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in decidual cells.
Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in decidual cells.
Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in decidual cells.

Anti-CASP2 Antibody

HPA050678
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCASP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CASP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID835
  • Target name
    CASP2
  • Target description
    caspase 2
  • Target synonyms
    CASP-2; caspase 2 apoptosis-related cysteine peptidase; caspase-2; ICH1; NEDD2; NEDD-2; neural precursor cell expressed developmentally down-regulated protein 2; PPP1R57; protease ICH-1; protein phosphatase 1, regulatory subunit 57
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42575
  • Protein Name
    Caspase-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysate, Lane 2: human 22RV1 whole cell lysate, Lane 3: human SGC-7901 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-2 antigen affinity purified polyclonal antibody (Catalog # A02384-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-2 at approximately 51KD. The expected band size for Caspase-2 is at 51KD.
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Flow cytometry data of 293T cells transiently transfected with HA-tagged caspase-2, fixed with 1% PFA, permeabilized with saponin and stained intracellularly with using anti-caspase-2, mAb (10C6) (Prod. No. AG-20T-0135) or positive control anti-HA.
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